A theoretical integrated Biophilic Streets Design Framework, is outlined and evaluated through the analysis of four street revitalisation projects from Vitoria-Gasteiz, Berkeley, Portland and Melbourne. Its practical applications and multiple urban benefits will be of value to street designers globally. The Biophilic Streets Design Framework demonstrated that the four case studies meet the main design categories, which is favourable since multiple additional benefits are likely to be obtained. Future research is needed to monitor and quantify the performance of biophilic streets design to address the increasing effects of climate change, environmental degradation and biodiversity loss in a cost-effective way.